control pcdna Search Results


pcdna 3.1-control  (Shanghai GenePharma)
90
Shanghai GenePharma pcdna 3.1-control
Pcdna 3.1 Control, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc06755201-58-18-28?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
pcdna 3.1-control - by Bioz Stars, 2026-07
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control pcdna  (Ribobio co)
90
Ribobio co control pcdna
Control Pcdna, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc05204041-40-11-18?v=Ribobio+co
Average 90 stars, based on 1 article reviews
control pcdna - by Bioz Stars, 2026-07
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pcdna-control vectors  (Shanghai GenePharma)
90
Shanghai GenePharma pcdna-control vectors
Pcdna Control Vectors, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc08221344-86-36-41?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
pcdna-control vectors - by Bioz Stars, 2026-07
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blank vector control (pcdna  (Ribobio co)
90
Ribobio co blank vector control (pcdna
Effect of circ_0001821 silencing on cell biological properties was counteracted by overexpression <t>of</t> <t>ISOC1.</t> A Overexpression efficiency of ISOC1 was examined by western blot assay. B – J SW620 and HCT116 cells were transfected with si-NC, si-circ_0001821, si-circ_0001821 + <t>pcDNA</t> or si-circ_0001821 + ISOC1. B and C The proliferative capacity of the cells was measured by colony formation assay and EDU assay. D and E The cell migration and invasion capabilities were assessed by transwell assay. F Flow cytometry was used to detect apoptosis of cells. G and H Glucose consumption and lactate production in CRC cell lines were tested. I and J Western blot assay was used to assess the protein expression of PCNA, MMP2, Bax and HK2. ** p < 0.01, *** p < 0.001
Blank Vector Control (Pcdna, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc09852123-63-9-17?v=Ribobio+co
Average 90 stars, based on 1 article reviews
blank vector control (pcdna - by Bioz Stars, 2026-07
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the blank control (pcdna)  (Shanghai GenePharma)
90
Shanghai GenePharma the blank control (pcdna)
PEG10 <t>regulates</t> <t>KIF2A</t> level by sponging miR-101-3p. ( A ) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. ( B ) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. ( C ) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with <t>pcDNA,</t> PEG10, si-NC, and si-PEG10. ( D ) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. ( E ) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P <0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.
The Blank Control (Pcdna), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc07523416-48-27-32?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
the blank control (pcdna) - by Bioz Stars, 2026-07
90/100 stars
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control pcdna  (GenScript corporation)
90
GenScript corporation control pcdna
PEG10 <t>regulates</t> <t>KIF2A</t> level by sponging miR-101-3p. ( A ) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. ( B ) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. ( C ) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with <t>pcDNA,</t> PEG10, si-NC, and si-PEG10. ( D ) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. ( E ) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P <0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.
Control Pcdna, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc08976346-55-14-19?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
control pcdna - by Bioz Stars, 2026-07
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control vectors (nc)  (Shanghai GenePharma)
90
Shanghai GenePharma control vectors (nc)
PEG10 <t>regulates</t> <t>KIF2A</t> level by sponging miR-101-3p. ( A ) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. ( B ) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. ( C ) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with <t>pcDNA,</t> PEG10, si-NC, and si-PEG10. ( D ) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. ( E ) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P <0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.
Control Vectors (Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc07655168-147-48-51?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
control vectors (nc) - by Bioz Stars, 2026-07
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control pcdna 3.1  (Promega)
90
Promega control pcdna 3.1
PEG10 <t>regulates</t> <t>KIF2A</t> level by sponging miR-101-3p. ( A ) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. ( B ) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. ( C ) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with <t>pcDNA,</t> PEG10, si-NC, and si-PEG10. ( D ) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. ( E ) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P <0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.
Control Pcdna 3.1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc05637157__mmc1-197-6-32?v=Promega
Average 90 stars, based on 1 article reviews
control pcdna 3.1 - by Bioz Stars, 2026-07
90/100 stars
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pcdna 6.2-gw/emgfp-mir-negative control  (GeneDireX Inc)
90
GeneDireX Inc pcdna 6.2-gw/emgfp-mir-negative control
PEG10 <t>regulates</t> <t>KIF2A</t> level by sponging miR-101-3p. ( A ) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. ( B ) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. ( C ) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with <t>pcDNA,</t> PEG10, si-NC, and si-PEG10. ( D ) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. ( E ) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P <0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.
Pcdna 6.2 Gw/Emgfp Mir Negative Control, supplied by GeneDireX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pm23389731-49-1-10?v=GeneDireX+Inc
Average 90 stars, based on 1 article reviews
pcdna 6.2-gw/emgfp-mir-negative control - by Bioz Stars, 2026-07
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matched control (pcdna  (Ribobio co)
90
Ribobio co matched control (pcdna
PEG10 <t>regulates</t> <t>KIF2A</t> level by sponging miR-101-3p. ( A ) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. ( B ) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. ( C ) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with <t>pcDNA,</t> PEG10, si-NC, and si-PEG10. ( D ) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. ( E ) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P <0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.
Matched Control (Pcdna, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/ppr0123595-52-17-23?v=Ribobio+co
Average 90 stars, based on 1 article reviews
matched control (pcdna - by Bioz Stars, 2026-07
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an empty control pcdna 3.1/+ plasmid (pc/c)  (Ribobio co)
90
Ribobio co an empty control pcdna 3.1/+ plasmid (pc/c)
Overexpressing CITED2 also restored cancer cell proliferation and chemoresistance in gastric cancer cells with FGD5-AS1 downregulation. (A) In sh-FGD5AS1-transduced SGC-7901 and MKN-28 cells, a mammalian expression plasmid containing the whole cDNA sequence of human CITED2 (pc/CITED2), or an empty control pcDNA <t>3.1/+</t> plasmid <t>(pc/C),</t> was transfected into cells. After 48 h, qRT-PCR was carried out to verify the transfection efficiency (* P < 0.05). (B) A 5-day MTT assay was carried out to compare the proliferation rates between SGC-7901 and MKN-28 cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05). (C) In double-infected SGC-7901 and MKN-28 cells, 5-FU chemoresistance was compared between cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05).
An Empty Control Pcdna 3.1/+ Plasmid (Pc/C), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pmc07432170-66-20-26?v=Ribobio+co
Average 90 stars, based on 1 article reviews
an empty control pcdna 3.1/+ plasmid (pc/c) - by Bioz Stars, 2026-07
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pcdna empty control  (Ribobio co)
90
Ribobio co pcdna empty control
Overexpressing CITED2 also restored cancer cell proliferation and chemoresistance in gastric cancer cells with FGD5-AS1 downregulation. (A) In sh-FGD5AS1-transduced SGC-7901 and MKN-28 cells, a mammalian expression plasmid containing the whole cDNA sequence of human CITED2 (pc/CITED2), or an empty control pcDNA <t>3.1/+</t> plasmid <t>(pc/C),</t> was transfected into cells. After 48 h, qRT-PCR was carried out to verify the transfection efficiency (* P < 0.05). (B) A 5-day MTT assay was carried out to compare the proliferation rates between SGC-7901 and MKN-28 cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05). (C) In double-infected SGC-7901 and MKN-28 cells, 5-FU chemoresistance was compared between cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05).
Pcdna Empty Control, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+pcdna/pm30372882-48-84-91?v=Ribobio+co
Average 90 stars, based on 1 article reviews
pcdna empty control - by Bioz Stars, 2026-07
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Image Search Results


Effect of circ_0001821 silencing on cell biological properties was counteracted by overexpression of ISOC1. A Overexpression efficiency of ISOC1 was examined by western blot assay. B – J SW620 and HCT116 cells were transfected with si-NC, si-circ_0001821, si-circ_0001821 + pcDNA or si-circ_0001821 + ISOC1. B and C The proliferative capacity of the cells was measured by colony formation assay and EDU assay. D and E The cell migration and invasion capabilities were assessed by transwell assay. F Flow cytometry was used to detect apoptosis of cells. G and H Glucose consumption and lactate production in CRC cell lines were tested. I and J Western blot assay was used to assess the protein expression of PCNA, MMP2, Bax and HK2. ** p < 0.01, *** p < 0.001

Journal: Biochemical Genetics

Article Title: High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis

doi: 10.1007/s10528-022-10262-z

Figure Lengend Snippet: Effect of circ_0001821 silencing on cell biological properties was counteracted by overexpression of ISOC1. A Overexpression efficiency of ISOC1 was examined by western blot assay. B – J SW620 and HCT116 cells were transfected with si-NC, si-circ_0001821, si-circ_0001821 + pcDNA or si-circ_0001821 + ISOC1. B and C The proliferative capacity of the cells was measured by colony formation assay and EDU assay. D and E The cell migration and invasion capabilities were assessed by transwell assay. F Flow cytometry was used to detect apoptosis of cells. G and H Glucose consumption and lactate production in CRC cell lines were tested. I and J Western blot assay was used to assess the protein expression of PCNA, MMP2, Bax and HK2. ** p < 0.01, *** p < 0.001

Article Snippet: Isochorismatase domain containing 1 (ISOC1) overexpression vector (ISOC1) and blank vector control (pcDNA) were also constructed by Ribobio.

Techniques: Over Expression, Western Blot, Transfection, Colony Assay, EdU Assay, Migration, Transwell Assay, Flow Cytometry, Expressing

PEG10 regulates KIF2A level by sponging miR-101-3p. ( A ) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. ( B ) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. ( C ) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with pcDNA, PEG10, si-NC, and si-PEG10. ( D ) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. ( E ) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P <0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Long Non-Coding RNA Paternally Expressed Imprinted Gene 10 (PEG10) Elevates Diffuse Large B-Cell Lymphoma Progression by Regulating Kinesin Family Member 2A (KIF2A) via Targeting MiR-101-3p

doi: 10.12659/MSM.922810

Figure Lengend Snippet: PEG10 regulates KIF2A level by sponging miR-101-3p. ( A ) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. ( B ) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. ( C ) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with pcDNA, PEG10, si-NC, and si-PEG10. ( D ) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. ( E ) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P <0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.

Article Snippet: SiRNA against PEG10 (si-PEG10) or KIF2A (si-KIF2A) and the negative controls (si-NC), KIF2A overexpression vector (KIF2A) and the control (pcDNA), PEG10 overexpression vector (PEG10) and the blank control (pcDNA) were purchased from Genepharma (Shanghai, China).

Techniques: Binding Assay, Sequencing, Luciferase, Activity Assay, Transfection, Reporter Assay, Quantitative RT-PCR, Western Blot, Real-time Polymerase Chain Reaction

Overexpressing CITED2 also restored cancer cell proliferation and chemoresistance in gastric cancer cells with FGD5-AS1 downregulation. (A) In sh-FGD5AS1-transduced SGC-7901 and MKN-28 cells, a mammalian expression plasmid containing the whole cDNA sequence of human CITED2 (pc/CITED2), or an empty control pcDNA 3.1/+ plasmid (pc/C), was transfected into cells. After 48 h, qRT-PCR was carried out to verify the transfection efficiency (* P < 0.05). (B) A 5-day MTT assay was carried out to compare the proliferation rates between SGC-7901 and MKN-28 cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05). (C) In double-infected SGC-7901 and MKN-28 cells, 5-FU chemoresistance was compared between cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05).

Journal: Frontiers in Genetics

Article Title: Long Non-coding RNA FGD5-AS1 Regulates Cancer Cell Proliferation and Chemoresistance in Gastric Cancer Through miR-153-3p/CITED2 Axis

doi: 10.3389/fgene.2020.00715

Figure Lengend Snippet: Overexpressing CITED2 also restored cancer cell proliferation and chemoresistance in gastric cancer cells with FGD5-AS1 downregulation. (A) In sh-FGD5AS1-transduced SGC-7901 and MKN-28 cells, a mammalian expression plasmid containing the whole cDNA sequence of human CITED2 (pc/CITED2), or an empty control pcDNA 3.1/+ plasmid (pc/C), was transfected into cells. After 48 h, qRT-PCR was carried out to verify the transfection efficiency (* P < 0.05). (B) A 5-day MTT assay was carried out to compare the proliferation rates between SGC-7901 and MKN-28 cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05). (C) In double-infected SGC-7901 and MKN-28 cells, 5-FU chemoresistance was compared between cells infected with sh-FFG5AS1 and pc/C, and those infected with sh-FFG5AS1 and pc/CITED2 (** P < 0.05).

Article Snippet: A mammalian expression plasmid (pcDNA 3.1/+) containing the whole cDNA sequence of human CITED2 (pc/CITED2) and an empty control pcDNA 3.1/+ plasmid (pc/C) were purchased from RiboBio (RiboBio, Guangzhou, China).

Techniques: Expressing, Plasmid Preparation, Sequencing, Transfection, Quantitative RT-PCR, MTT Assay, Infection